P01.22. Identification of a peptide biomarker from bromelain, an extract of ananas comosus merr, using LC-SRM/MS
نویسندگان
چکیده
Methods Stem Br (Vital Nutrients lot # 2890; Middletown, CT), was tested for authenticity and purity. Female C57BL/6J mice, (Jackson Laboratory; Bar Harbor, ME) received one bolus, i.p. injection of Br (12mg/kg) in 0.5ml of physiological saline and plasma collected at baseline 3, 6, 12 and 24 hours. All procedures were approved by the Animal Care Committee at University of CT Health Center. Post SDS-PAGE in gel digestion, samples (Br raw material, spiked plasma and plasma from i.p. teated animals) were analyzed via LC/MS/MS (Liquid ChromatographyMass Spectrometry-Mass) and LC-SRM/MS (Liquid Chormatography-Selected Reaction Monitoring/Mass Spectrometry). Data were searched using Mascot and Scaffold algorithms.
منابع مشابه
LC-MS/MS Identification of a Bromelain Peptide Biomarker from Ananas comosus Merr
Bromelain (Br) is a cysteine peptidase (GenBank AEH26024.1) from pineapple, with over 40 years of clinical use. The constituents mediating its anti-inflammatory activity are not thoroughly characterized and no peptide biomarker exists. Our objective is to characterize Br raw material and identify peptides in the plasma of Br treated mice. After SDS-PAGE in-gel digestion, Br (VN#3507; Middletown...
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